CD8+ NFAT-Luc Jurkat
CBP74608
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| I. Background | |
| CD8+(或CD8阳性)是T细胞表面的一种共受体糖蛋白,主要作为T细胞亚群的表面标志物。它由CD8A和CD8B基因分别编码的α和β链组成,以αα同源二聚体或αβ异源二聚体的形式存在。该分子是MHC I类分子的受体,能增强T细胞受体(TCR)与抗原肽-MHC I类分子复合物的结合亲和力。因此,CD8分子是鉴别和界定细胞毒性T细胞的核心表型标志。 | |
| II. Description | |
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CD8+ NFAT-Luc Jurkat 报告基因药靶模型很好的模拟了体内 CD8+ 的信号转导过程。 |
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| III. Introduction | |
| Expressed gene: | CD8+ |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+800μg/ml Hygromycin B+10μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
Figure 1.Recombinant CD8+ NFAT-Luc Jurkat stably expressing CD8a&CD8b.
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly recommend purchasing from Cobioer). Dose Response of CD3 Antibody in CD8+ NFAT-Luc Jurkat(C40).